Riya ..

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Questions related from Riya ..

I'm doing RNA Slot blot assay for 18S rRNA detection but I'm struggling with the results. What could be the reasons for negative results?

I am performing RNA slot blot assay wherein I blot total RNA on Hybond + Nylon membrane. I have designed probes against the 18s rRNA. I am carrying out hybridization of the RNA and labelled probe...

21 September 2022 5,809 1 View

Hybridization Probe calculation: How I can prepare 10 ng/ul of probe working stock?

Can anyone please help me to prepare a probe working solution of10 ng/ul for my hybridization experiment? I have 100uM stock (Made by diluting 36.26 ug in 50 ul sterile water) [Total nmole= 5...

21 September 2022 5,339 0 View