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I recently checked and found that my KHYG-1 cell line shows 2 positive peaks for an intracellular protein that I am trying to study. I would expect any cell line to be homogeneous in this sense....
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I am trying to isolate single cells from mouse lung with B16F10 metastatic tumours. Typically, after enzymatic digestion, I filter the cells through 70um filter, followed by 40% Percoll to remove...
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