Ritwika Basu

2 Questions 2 Answers 0 Followers

Questions related from Ritwika Basu

Experiencing difficulties with phiC31 integrase mediated gene inversion experiment. What might be the possible reasons??

I am currently attempting to invert my reporter gene, which is present in an inverted orientation between PhiC31 attB and attP sites. However, when I performed the gene inversion experiment using...

28 May 2025 3,521 3 View

Experiencing difficulties with cloning of super primer PCR products. Why isn't the desired insert being incorporated into the vector?

I am attempting to incorporate two restriction enzyme sites into a plasmid vector (p15A origin PREGFP) using super primer PCR. My desired insert length is 80 bp, containing one restriction sites...

17 December 2024 8,175 0 View