Ritu Rathi

2 Questions 6 Answers 0 Followers

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Protein is not entering into gel (8%) from wells after DSS cross-linking?

Hi everyone, I am trying DSS crosslinking for my protein. My protein is expected to make trimer or hexamer with Ub. To prove this, I am crosslinking my protein with DSS. I tried crosslinking for...

24 September 2017 4,632 6 View

How to connect Superdex 200 column for reverse-flow in AKTA prime plus system?

I want to use flow path in up-flow mode. So how can I do it without reconnecting column?

03 July 2017 8,301 2 View