Rithika Ashokan

4 Questions 4 Answers 0 Followers

Questions related from Rithika Ashokan

Why two bands are seen in sds after thrombin cut ?

I have purified my protein using Ni-NTA and kept for thrombin cut ( buffer 25 mM tris, 50 mM Nacl, 5 % glycerol , 10 uL thrombin pH 7.9 kept magnetic stir over night. and next dat i centrifuged...

16 January 2025 4,071 3 View

Regarding protein crystallization. what happens if i change the ph of protein from 7.9 to 7.5 ?

I have purified protein using affinity and ion exchange, in both of the buffers i used ph of 7.9, while concentrating in buffer exchange, i changed the ph to 7.5 and concentrated upto 5.4 mg/ml...

10 January 2025 7,362 3 View

Purifying co-complex protein ?

i am trying to purify co-complex protein. two plasmid has been transformed in a single e coli (Rosetta com cell ). Then did primary and secondary culture induced IPTG ( 0.25mM) at 0.5 OD (600nM)....

12 December 2024 7,643 3 View

Why the sonication is not happening properly ?

i am trying to lysis the bacterial pellet (500ml culture pellet) with 50ml of lysis buffer ( 0.5 m Tris HCL, 5M NaCl pH 8.0, 100mM PMSF, Lyzozyme ) by sonication (Amp 20, Esp time 10 mins,...

21 October 2024 358 3 View