How can I prevent interference of IgG heavy chain when pulling down proteins by IP?

The cell lysis buffer I am using is: 500mM Tris PH8 200mM NaCl 0.1mM EDTA 0.5% NP40 10% Glycerol with protease inhibitors. The problem is that i had to use the same anibofy for both IP and...

15 November 2016 9,567 3 View