Does HEK293T cells have cas9 enough to be used in CRISPR screening instead of adding Cas9 plasmid separately?

I intend to do CRISPR screening on a cell line made in HEK293T cells and I do have Whole Genome CRISPR library (Brunello) plasmid .; this plasmid has 17441 SgRNA targeting almost 900 non essential...

27 January 2022 1,489 3 View