Rini Yanti

3 Questions 5 Answers 0 Followers

Questions related from Rini Yanti

What happens in this tricine gel ?

I got this SDS PAGE gel after running about 3 hours with tris tricine buffer system. Half of the gel showed the separation of protein, but the others showed the blank band. I'm not sure wether...

20 February 2015 527 3 View

How can I estimate the molecular weight if the equation of curve is not linear ?

I run the marker and unknown protein, and i got the very clear band of the marker. The molecular weight of the marker are 10 - 250 kda. After making a graph for rf vs log MW, the equation is not...

30 December 2014 9,467 7 View

Do these bands separate well or do I need to re-run the samples with another method?

There are same thick bands of unknown protein. I'm not sure whether this band because of containing of salt or because of the low of molecular weight. I need some discussion or sharing experience...

10 December 2014 1,510 6 View