Riley Lin

4 Questions 1 Answers 0 Followers

Questions related from Riley Lin

Is it possible to observe M+2H in positive mode lc ms?

Hi im wondering if its possible that my compound gets protonated twice resulting in M+2H for positive mode? I ran my sample in positive mode and was supposed to observe m/z of 593.06xx but instead...

04 August 2022 7,972 0 View

Sequence obtained from rapid cDNA ends amplification did not align with the computed sequence?

recently i have been trying to amplify a p450 gene but it kept failing. I was able to amplify a small fragment of the gene but never the whole gene so i decided to go for RACE experiment. With...

21 November 2021 7,058 3 View

Can anyone recommend me books or links useful for a newbie in genetics?

Im recently working on cloning and stuck with PCR amplification and im having a hard time with snapgene and all - -the plasmid construction, the primer design Could anyone recommend me what...

08 September 2021 9,078 3 View

Getting a pcr band larger than expected, why?

Hi, im trying to amplify the gene of size 1.4 kb, however, i ran the gel and found the band of size apx 1.6 kb instead? Can anyone please explain to me the possible reasons behind this?

08 September 2021 1,230 1 View