My protein is a recombinant protein constitutively expressed in Pichia.Why am I getting a smear/ dragging of the loaded protein?

The protein (55KDa) has low contentration. It is purified via ammonium sulphate salting out and desalted with pD10 column. Will working with SDS-PAGE (10% resolving and 5% stacking), a drag is...

06 June 2019 3,460 15 View