Ricky Lali

5 Questions 1 Answers 0 Followers

Questions related from Ricky Lali

How to interpret digital PCR scatter plot?

Hello everyone, I am unfortunately getting sub-quality results from my dPCR runs where the clustering (i.e. fluorescent amplitude) of the FAM and VIC assays are not separated. Essentially, I am...

03 March 2018 1,247 4 View

Which gene expression database is best for obtaining baseline expression for any gene?

Hello everyone, I am quite new to gene expression analyses and I was wondering which database would be suited towards finding the level of expression of a particular gene in a given tissue. For...

04 April 2016 1,831 1 View

Are there any annotation tools that allow you to determine the coding length of genes?

Hello everyone, Does anyone know of an annotation tool (perhaps within dbNSFP) that outputs the total coding length (in base pairs) of genes? Any help is appreciated. Thank you.

01 January 2016 738 2 View

Does anyone have experience with haplotype sharing analysis?

Hello, I have both sequencing and genotype data for a small population and was wondering how to go about analyzing whether any of these individuals have a shared haplotypes along a particular...

10 October 2015 2,922 2 View

Why does the second elution fraction (out of 3 total) contain the greatest amount of target protein?

I ran a hexa-his tagged protein through Ni-NTA column and used elution buffer (250mM imidazole) to elute the purified product. After running on SDS PAGE, I found that the second elution fraction...

04 April 2014 6,184 2 View