Can anyone help me, I am having a difficult time trying to clone my small ~500bp gene sequence into pRU1101 & pRU1105 plasmids?

I am cloning into in-lab made electrocompetent DH5a cells. The best I can get in transformation efficiency is Yx10^8. I tested this with pUC19.  I have added unique restriction endonuclease...

03 July 2014 3,045 3 View