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Questions related from Richard A Kahn
We are seeing decreases in the %'age of transfected cells (HeLa, COS7, MEFs) with time of storage over a few weeks, of purified plasmids at 4C. Seems more stable when stored at -80C. I don't think...
09 September 2015 8,895 4 View
Does anyone know of an example of a gene that is essential to mouse embryo fibroblasts (MEFs), in that cells die when treated with Cre recombinase that leads to deletion of the gene. But it is not...
12 December 2014 8,788 4 View
For years we have had occasional need to permeabilize cells prior to fixation and later use in immunofluorescence studies. This lowers cytosolic staining and increases our ability to see some...
09 September 2014 5,343 9 View
Members of my group are trying to generate cell lines carrying inducible siRNAs. We are using the pLKO-TetON plasmid, into which we have cloned multiple sequences that we know have worked as...
02 February 2014 6,884 10 View
