Richard Barker

6 Questions 13 Answers 0 Followers

Questions related from Richard Barker

What is the best & easiest way to design QPCR primers for Arabidopsis genes?

I have some Arabidopsis transcripts that i'd like to investigate with QPCR. I've looked at few webs sites and papers but am still unsure what is the best & easiest way to design primers for...

09 September 2017 1,067 3 View

Will my DNAse treatment breakdown all the DNA if I add the EDTA before the 37C incubation?

I am currently using Ambion DNAse to remove any DNA contamination from my RNA samples. The protocol requires a 37C incubation for 30min and then a 75C incubation for 10min to deactivate the...

09 September 2013 8,484 5 View

How do you quantify a microRNA?

I've read various papers on how to quantify microRNA by stemloop PCR and other QRT-PCR machine based methods but none ever explain how to design the primers or other essential details.

08 August 2013 2,470 5 View

How does one over express a microRNA?

I've identified some microRNAs that I think are interesting. I would like to over express them with a 35S promoter but don't know where to begin? I have a destination vector with the promoter, so...

08 August 2013 9,265 5 View

I have some Arabidopsis genes that I'm interested in, is there a way to easily identify if they are in a family or have any homologues?

I've tried blasting the translated sequence that provides some information, i've looked at the TAIR website (http://www.arabidopsis.org/) and the biofuel feedstock genomics resource which produced...

03 March 2013 8,717 5 View

How many Arabidopsis root tips will be needed to generate 25 µg total RNA, so that the mRNA can be used for RNAseq? Will this be the same for miRNA?

I want to create RNA and miRNA libraries for the Arabidopsis root tip and was wondering how many plants i should grow during my experiment?

04 April 2012 3,357 10 View