Can a CTAB/PVP RNA extraction buffer be reused multiple times with cycles of refrigeration and heating or should it be made up fresh?

I am currently extracting RNA from whole wheat grains at different developmental stages using a CTAB extraction buffer containing: 2% CTAB, 2% PVP, 100mM Tris-Hcl , 25mM EDTA, 2M NaCL and 0.5g...

28 November 2016 1,764 4 View