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Questions related from Richard Allen
I have been having issues with my THP-1 cells for most of this year - when I try to differentiate them with PMA into macrophage-like cells about 1/3 of the cells do not adhere properly (either...
30 October 2023 2,343 2 View
Hi everyone, I am experiencing an issue with my THP-1 cells. After addition of 100 ng/ml PMA to differentiate into macrophage-like M1 cells, the THP-1s are not fully adhering down. There is a...
13 April 2023 7,349 2 View
I am using β-actin as a loading control for my western blots to enable quantification of proteins. I never used to have an issue with this protein but recently I am getting faint bands during...
17 January 2023 1,843 4 View
