I'm trying to polarize THP-1 and J774a.1 macrophages into M1 and M2 subtypes. However, I see massive differences in protein loading? any insights?

I'm trying to polarize THP-1 and J774a.1 macrophages into M1 and M2 subtypes. I lyse the polarized cells in RIPA buffer and quantitate proteins using BCA assay. However, I always see stark...

09 April 2025 4,101 2 View