Ricardo A. Parra-Cruz

3 Questions 1 Answers 0 Followers

Questions related from Ricardo A. Parra-Cruz

Why do we measure esterase activity in carbonic anhydrase at 348nm instead of 405nm?

Hi knowledgeable beings, I am still grappling with the fact that people assay pNPA hydrolysis at 348nm. As far as, I understand esterase activities are always measure at 400 or 405 nm, but this...

06 June 2017 6,543 1 View

How to manually set a disulfide bond in a pdb file?

For the past month, I have been trying to model a tetrameric protein-ligand with a charmm forcefield. Noteworthy is that this protein is held together by disulfide bridges (ssbond). the dynamics...

07 July 2016 8,072 4 View

How can I use CHARMM36 for a metallloprotein?

I am currently using three different versions of gromacs 5.0; 4.6.5 and 4.5. In all of them, I have been trying to run the CHARMM forcefield. Because it recognizes HETATOM - ZN. However, every...

03 March 2016 8,485 4 View