Why MiFish Primers amplify when they're alone but not when they have an overhang sequence (Illumina adapter + tag)?

Good day everyone. I'm working with eDNA Metabarcoding for freshwater fishes in Brazil, I ran tests amplifying positive controls and samples with the primer MiFish-U (Miya et al 2015) alone (no...

12 January 2024 5,071 2 View