Rhudith Cabulong

4 Questions 11 Answers 0 Followers

Questions related from Rhudith Cabulong

How can I minimize background colonies during gene deletion?

Im currently doing gene deletion. I am following the procedure in openwetware. During my first 3 gene deletion I got high efficiency, however, in my 4th deletion now, I got very very low...

02 February 2016 7,384 7 View

Why is my HPLC baseline not stable?

We have back-flushed our HPLC using methanol and then we purged it with 25mM and 5mM sulfuric acid. We have been purging it for 4 days already using 5mM sulfuric acid but still the baseline is not...

04 April 2015 4,951 8 View

What could be the reason for no Protein Expression after cloning?

I inserted my gene in pACYCduet-1 MCS2, sequenced the construct and the sequence was fine. Everything was OK. However, when I tested its expression by running SDS-PAGE, the result was negative. I...

03 March 2015 6,429 14 View

My cloning is unsuccessful, I repeated and varied my variables yet nothing happened. Can I still get empty vector transformants. What should I do?

I'm using pACYC vector amd I'm doing double digestion with BamHI and HindIII for both of my vector and insert. 1) In my primer design, I only added one base pair before the restriction site...

11 November 2014 5,485 5 View