Why is there no blue color development after addition of TMB substrate during ELISA assay?What could be some of the reasons that would cause this?

Am doing a pilot run of Sandwish ELISA using the R&D Systems DuoSet ELISA kit. There's no substrate provided in this kit, so I use one from BD. I prepare the sustrate 15mins before addition to...

05 May 2018 3,787 3 View