Rhiannon Jamieson-Williams

3 Questions 1 Answers 0 Followers

Questions related from Rhiannon Jamieson-Williams

How to calculate delta-delta CT, and standard deviation, when using biological replicates?

I have 3 experimental conditions, as well as a control. Each of these 4 conditions has 3 biological replicates. When performing the qPCR, each biological replicate was run in triplicate (technical...

03 March 2018 2,699 6 View

What is the sequence for the HSV-tk gene used in homologous recombination vectors such as pPNT?

I can't seem to find the sequence of the HSV-tk component of these types of vectors. Of course, the full sequence of the vectors are available online, but none that are annotated in a way that...

12 December 2016 428 1 View

Ligated 1.4kb gBlock into pBluescript II KS+. Colonies and liquid culture grows fine, but gel shows low level of DNA. Sequencing faulty. Help?

I am working on setting up a CRISPR/Cas9 experiment. I have successfully cloned my sgRNAs, but due to the size of my deletion, will need to use a vector template instead of an ultramer. The...

05 May 2016 8,276 3 View