Rhiannon Jackson

2 Questions 2 Answers 0 Followers

Questions related from Rhiannon Jackson

Help with primer calculations?

I am setting up an RT-PCR experiment for the first time and I am trying to get the calculations for making up my working primers. This is what I have so far. Forward primer: initial...

26 May 2022 869 1 View

Nested PCR: band in second amplification?

I have been getting a band in the second (nested) amplification of the negative from the initial PCR, but not in my negative control testing for contamination. Any ideas? Information: I am using...

09 March 2022 3,733 2 View