Reza Kh

3 Questions 10 Answers 0 Followers

Questions related from Reza Kh

I use AQ97 High Fidelity DNA Polymerase enzyme and rice DNA for PCR but the gel bands are fade, how can i improve the yield?

30μl reaction components include, DNA 2μl, Master 15μl, Primer 0.7+0.7μl, water 11.6μl PCR program 98°C 2min 1 98°C 15S 35 56-61°C 20S 35 72°C 30S 35 72°C...

04 December 2024 6,821 11 View

How can i use hexamethyldisilazane(HMDS) for drying instead of Co2 and freez drying for SEM analysis of rice anther and pollen?

could you please help with a protocol? should i do it directly on the aluminium stub after dehydration with 100% alcohol or not? should i release the pollen or whole anther is better and dehisce...

04 June 2024 4,878 8 View

How to prepare 100ml solution of 2.5% Glutraldehyde in 0.1M PBS(phosphate buffer) pH about 7, from 25% Glutradehyde and 1X PBS?

final solution volume is 100ml

04 June 2024 501 0 View