Revathy Nadhan

2 Questions 1 Answers 0 Followers

Questions related from Revathy Nadhan

Can anyone help with the absolute quantification Real Time PCR analysis to analyze the ct values obtained and convert them to the expression levels?

I had 10 different tissue samples, of which 5 were normal subjects and 5 were diseased. I have ran a AQ RT PCR for my gene along with GAPDH and I have the ct values. But how to analyse expression...

04 April 2015 3,006 7 View

Is there anyone working with the trophoblastic tumors or placenta for selecting appropriate loading control for the western blotting?

I am not sure of the variations that I get with the loading controls during western blot. Is there any other proteins than beta actin which have been used stably now with you?

01 January 2014 1,304 3 View