Revathy Krishanmurthi

2 Questions 6 Answers 0 Followers

Questions related from Revathy Krishanmurthi

EMSA kd interpretation?

I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...

25 September 2015 5,444 3 View

Any suggestions on protein aggregates during buffer exchange in size exclusion?

Hi fellow researchers, am an newbie in protein purification and hence this question. I am working on a novel protein whose size is 17kda.it does not have cysteine. It's PI is around 7.83. I did a...

21 June 2015 2,776 5 View