Reshma Ramesh

3 Questions 3 Answers 0 Followers

Questions related from Reshma Ramesh

Does anyone know how to prevent protein coeluting with other impurities in size exclusion Column?

I have been trying to purify a transcription factor. Protein has MBP and His Tag. I use His TRAP as my first purification step and then after I do size exclusion, but my protein (83 KDa) elutes...

24 April 2023 9,183 5 View

Does anyone know how to prevent protein coeluting with other impurities in size exclusion Column

I have been trying to purify a transcription factor. Protein has MBP and His Tag. I use His TRAP as my first purification step and then after I do size exclusion, but my protein (83 KDa) elutes...

01 January 1970 3,673 5 View

Protein showing higher 260/280

I am purifying a protein by denaturation method(8M Urea). After solubilizing and running a His TRAP with 1M NaCl Wash, and elution. I refold by dialyzing to 2M urea and then desalting the column...

01 January 1970 2,972 5 View