Renzo Cortez

5 Questions 1 Answers 0 Followers

Questions related from Renzo Cortez

How can I measure metformin in plasm and urine?

I need to determine the levels of metformin in plasm and the rate of excretion as well. Does it could be assessed by spectrophotometry? Or Are there other methods more reliable?

06 June 2018 5,857 5 View

A protocol to perform a overlap extension PCR (OE-PCR)?

I need to join 3 separate PCR products for sequencing. I have read that OE-PCR is used mainly for mutagenesis, but also to join PCR products, however I don't find reference articles to apply it...

04 April 2018 757 10 View

What should I do if my primers are amplifying two equal sequences (a gen and a pseodogene)?

I´m trying to amplify two exons of CYP2D6, but I have been obtaining inespecific bands. I have aligned the sequences of CYP2D6 and CYP2D7 (a pseudogene with similar sequence) with my primers and...

11 November 2017 4,064 4 View

What is the principle of methanol:Chloroform protocol for protein precipitation?

I need to know the chemical principles in protein precipitation.

11 November 2017 7,559 4 View

I need a protocol for DNA extraction from oral swabs, would you recommend me one please?

I am looking for a protocol for DNA extraction from bucal swabs or saliva but most of them use kits and I only have reactives for traditional methods.

07 July 2017 7,095 5 View