Renuka Harit

2 Questions 1 Answers 0 Followers

Questions related from Renuka Harit

Recombinant protein has 30 Cys residues, performed ellman's assay to detect free thiol groups the OD came close to blank ?

Dear Researchers, My protein of interest has 30 cys residues in it. After refolding, all form disulfide bonds. I performed Ellman's assay to detect the free thiol groups in the protein. The OD of...

25 November 2024 6,882 0 View

Hello Scientists, RFU is negative in trypsin and fluorogenic substrate Z-RR-AMC fluorogenic assay What is the reason?

I am performing fluorogenic assay with trypsin and fluorogenic assay Z-RR-AMC, my readings are decreasing with increasing time. What could be the reason for this strange results. Please...

02 April 2024 4,720 1 View