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Questions related from Renu Verma
Dear All, I have set up a 5-plex qPCR. The multiplex works fine but the PCR efficiency doesn't look that great. I am throwing in 250ng human genomic DNA and I get Ct in range of 30-35 for all my...
14 February 2020 7,345 3 View
Dear All, I am looking for a kit to extract total RNA from MTB which I will later use for RT-qPCR. My initial sample would be very low and cannot afford to loose RNA during the extraction. Also,...
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Hi All, I am trying to perform melt curve analysis for a single gene using Taqman probe. I performed asymmetric QPCR on StepOne Plus. Even though I got the melt curve peaks, they are not as...
18 January 2019 2,246 9 View
Will heat killing at 95 degree Celsius will lyse the mycobacteria or just kill it ? what if I heat kill the MTB and store it at -20 for a week and then perform DNA isolation? Will the nucleases...
28 December 2015 5,111 12 View
I am using HRP conjugated antibody.
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