Renerio Jr. Pelegrino Gentallan

3 Questions 7 Answers 0 Followers

Questions related from Renerio Jr. Pelegrino Gentallan

Does freeze drying and storing plant tissues in -80 deg. C affect the total RNA yield in RNA extraction?

It is because I have very low RNA yield. I was thinking if it is because I use 1-week old freeze dried samples stored in the freezer at -80 degree celsius. Amybe it is better to use fresh samples...

02 July 2014 7,601 9 View

In spectrophotometric analysis, what would a >2.0 260nm/280nm absorbance ratio of an RNA extract indicate?

My hypothesis: Since nucleic acids has a peak absorbance at 260nm wavelength and proteins at 280nm, the ratio of greater than 2.0 is considered relatively pure RNA. Twist: However, literature...

27 June 2014 1,595 4 View

I have 62 multiple aligned sequences. How can I generate a few common primers that will amplify the 62 sequences?

I am currently using genetyx and clustalw for alignment.

11 June 2014 7,246 7 View