Renerio Jr. Pelegrino Gentallan

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Questions related from Renerio Jr. Pelegrino Gentallan

Does freeze drying and storing plant tissues in -80 deg. C affect the total RNA yield in RNA extraction?

It is because I have very low RNA yield. I was thinking if it is because I use 1-week old freeze dried samples stored in the freezer at -80 degree celsius. Amybe it is better to use fresh samples...

02 July 2014 7,592 9 View

In spectrophotometric analysis, what would a >2.0 260nm/280nm absorbance ratio of an RNA extract indicate?

My hypothesis: Since nucleic acids has a peak absorbance at 260nm wavelength and proteins at 280nm, the ratio of greater than 2.0 is considered relatively pure RNA. Twist: However, literature...

27 June 2014 1,585 4 View