Renee Tamming

2 Questions 2 Answers 0 Followers

Questions related from Renee Tamming

Help with extraction of RNA from synaptoneurosomes?

Hi, I am trying to purify synaptoneurosomes for RNA analysis from 4-week mouse brains. I am using a Percoll density gradient followed by washing 2X in buffer to remove residual Percoll (Dunkley...

20 August 2019 8,295 3 View

Cells dying during culture of mouse embyronic hippocampal neurons?

Run-down of my protocol: Dissect hippocampus into 500ul ice cold dissociation media (HBSS +sodium pyruvate, glucose, 10mM HEPES). Wash 2x with dissociation media. Add 450 dissociation media +...

15 March 2017 5,632 4 View