Remi Joe

3 Questions 2 Answers 0 Followers

Questions related from Remi Joe

Western Blot Transfer Band Smearing?

Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.

30 July 2020 9,480 9 View

Failed gateway cloning?

Hi everyone, I am having a few doubts about gateway cloning. We are using gateway cloning for protein expression using pDEST15 as our destination vector. I attached a picture of the pDEST15...

11 March 2020 7,623 2 View

How to make a solution of 5% formalin, 5% triton-x100, 1% KOH?

I am looking to make a fixative per the protocol of Sakata-Haga et. al for bone staining. It calls for a fixative solution of 5% formalin, 5% triton and 1% KOH. I can buy 10% neutral buffered...

28 February 2020 4,665 3 View