What is the minimum size of PCR product: tRNA, that needs to be cloned and then sent to sequencing in order to get accurate sequencing?

Hi, I need to amplify a tRNA fragment in a normal PCR, The primers I designed suppose to give me a product of 40 base pairs. I want to transform it into pGEM and send it to Sanger sequencing . My...

04 May 2023 5,315 3 View