Reham M Milhem

3 Questions 9 Answers 0 Followers

Questions related from Reham M Milhem

How can I tell if my vector can be used for transient transfection in mammillian cells or for stable transfection?

I have two cDNAs; one cDNA is in a pCMV-sport6 and the other in a pCMV5 vector. I wish to begin a stable cell line with either HEK293 cells or HeLa cells. However, both vectors do not have a Neo...

09 September 2013 2,137 3 View

Concentration of plasmid for stable transfection.

I am using FugeneHD and wish to start a stable transfection using HeLa cell lines. As a starting point, what would be a good concentration of plasmid to transfect HeLa cells with?

09 September 2013 1,391 8 View

Starting a stable cell line?

I need to start a stable cell line using HEK293T cells, antibiotics, serial dilution and random integration over time. I have never seen the procedure but I read about it, however it is still...

08 August 2013 8,317 3 View