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Questions related from Rees Ross
I loaded the 1.5% agarose gel as standard. I loaded 6uL from PCR and 1uL loading dye. Why is the solution not sinking to the bottom of the wells?
24 October 2023 3,885 2 View
The first lane is 1kb ladder, the second lane is the ligation reaction, the third lane is plasmid only control. Previously the same PCR product gave clean bands but now it isn't and I don't know why.
03 October 2023 2,354 1 View
My supervisor suggested that the bp of PCR products is related to the digestion time. I cannot find anything on the internet to solve my problem. I have a PCR product 500bp. My supervisor implied...
28 September 2023 2,889 3 View
I have run PCR and digestion (Not1-hf & Nde1) that gave good bands. The plasmid was PET-28-make123 and the PCR product was franken-flag-dsred. Then I performed ligation and ran a gel with...
22 September 2023 8,628 3 View
