Is it feasible to do cell viability test by counting cells using trypan blue in a 96-well plate? How can I detach cells from wells exactly?

I will use MCF-7 cell line, so after treatment and incubation, do I need to remove media, wash with PBS, add trypsin and new media then take a sample and mix with trypan blue? and how much uL of...

03 January 2016 5,392 6 View