Rebecca Davies

3 Questions 4 Answers 0 Followers

Questions related from Rebecca Davies

How do I prevent PBMCs from sticking to MSCs in co-culture?

Hi all, I'm currently doing co-culture of MSCs and PBMCs in a 96 well plate. MSCs are left to adhere for 24 hours, before adding CD3/CD28 activated PBMCs (1:10 ratio). However, I find my...

18 February 2022 5,186 3 View

Why do my PBMCs die upon activation - how can I fix it?

Hello, I'm seeking guidance on how to activate my PBMCs without them dying. I have PBMCs frozen down in liquid nitrogen (primary PBMCs, either human or murine sourced - frozen down after...

07 December 2021 3,675 3 View

Can anyone help with cell identification by morphology?

We have been culturing MSCs from knee bone chips in which the MSCs migrate out into the culture flask. These have been taken from someone else in our group and resurrected from frozen (P3-4)....

08 January 2020 8,276 3 View