Rebeca Pallares Vega

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Questions related from Rebeca Pallares Vega

Is it possible to preserve Clostridia spores at -80C in water without glycerol?

I wondered if someone had ever stored spore suspension at -80C just in water or a saline solution without any glycerol or cryopreservation agent. We would like to transfer a protocol to LMICs to...

09 October 2024 705 1 View

Is it possible to use Overhangs with large different Tm with NEB Builder HiFi DNA assembly?

Dear cloning community, Is it possible to use Overhangs with large Tm difference with NEB Builder HiFi DNA assembly? 5´ 26 bp Tm 57ºC 3´ 24 bp Tm 72ºC Unfortunately, extending the 5´ doesn't...

18 June 2024 8,598 2 View