Razaul Haque

5 Questions 6 Answers 0 Followers

Questions related from Razaul Haque

How to adjust the scale of "0.0" of X-axis and Y-axis of from the same point in AUC curve R?

Hello everyone, I am facing a problem when making a plot in "R". I am generating a ROC curve, but in the graph I have been oberving that my "0.0" scale of of X-axis is far from "0.0" scale of...

26 May 2024 347 1 View

What is the optimum duration for short term B cell Culture?

Hello Everyone. Greetings, I am planning to perform short term B cell culture (without any stimulation) from ovarian cancer patient (source of B cell PBMC and Ascites). I don't understand how...

23 October 2020 5,851 3 View

How to improve cell viability during PBMC isolation?

Dear all, I am isolating PBMC from human blood over Ficoll . I am using 2mM EDTA as a washing buffer. after collecting buffy coat, I washed PBMC 2 times with 2mM EDTA. During washing I set...

24 June 2019 9,481 4 View

Is there any RNA isolation kit for human PBMC that yield good amount of RNA?

Hello Everyone, I am trying to extract total RNA human PBMC of healthy person. I used TriZole for cell homogenization. I used 2 x 10^6 no cell. But the RNA yield was very low. I got only 494 ng...

05 November 2018 2,760 5 View

How much time it is needed to activate T cell with IL-2 before thymidine incorporation ?

Hello everyone, I am working on T cell proliferation assay using 3H-thymidine. I am using PBMC to culture for 3 days (72 hourse). I am using IL-2 for T cell activation. Can any please tell me,...

12 March 2018 2,902 4 View