Ravindra Singh Rawat

6 Questions 10 Answers 0 Followers

Questions related from Ravindra Singh Rawat

How to separate Adenosine and Inosine?

Hi everyone I have been trying to resolve adenosine and Inosine on HPLC column. The details are as follows: 1) Column dimension: 150mmX4.6mm, with 5 micron bead size. 2) Mobile phase: 20mM...

08 August 2019 9,792 3 View

How much protein required?

Hello everyone What concentration of protein is usually required for DLS study for a single run?

05 May 2019 1,186 5 View

Why is protein not expressed?

Hi everyone! I am trying to express a bacterial protein which I have cloned in pet28a+ vector with NcoI site and XhoI site with C-terminal his-tag. The bacterial host I am using is BL21DE3 plysS....

04 April 2018 6,598 6 View

Is cryo EM a suitable technique for my prtoein?

Hi everyone! I have been working on a protein with mol. wt. of 45 kDa. This protein is present in inclusion bodies, which I have refolded and purified. I wish to carry out cryo EM experiments...

10 October 2017 5,380 3 View

Is ELISA reliable?

Hi Everyone! I'm trying to assay the binding activity of my protein against a cytotoxic protein. . I can do it in two ways, first by rescue assay of mammalian cell line and second by ELISA....

01 January 2017 8,211 7 View

Why is the expression of protein not consistent?

Hi everyone! I have cloned gene in pRSETB vector and expressed in BL21(DE3) host. After fresh transformation protein is expressed. However, there are following difficulties: 1) Even only one week...

10 October 2015 4,392 12 View