Ravikanth Nanduri

2 Questions 6 Answers 0 Followers

Questions related from Ravikanth Nanduri

Cells were slow growing after lentivirus transduction and puromycin antibiotic selection. Any suggestions to grow them better?

I have tried growing cells with low concentration of antibiotic. Initially I have used 1-2 ug/ ml concentration of puromycin for selection

18 December 2017 6,170 2 View

Do we need to do antarctic phosphatase treatment while we are using two different restriction enzymes to prevent self ligation of plasmid?

Do we need to do gel extraction before proceeding to ligation step with insert? My understanding is that antarctic phosphatase treatment is mandatory while we are digesting plasmid with single...

10 June 2017 2,930 2 View