Ravi Lokwani

4 Questions 10 Answers 0 Followers

Questions related from Ravi Lokwani

When we report MFI in manuscript or any document, so we mention MFI of positive population only or do we mention MFI of entire population?

There are few antibodies like CD54, CD88, for which only a fraction of my neutrophil population is positive and not all. So should I report the MFI of only positive population or MFI of entire...

02 February 2018 4,741 3 View

Does fixing of cells after staining with surface antibodies for flow cytometry affect the expresion (MFI)of antibodies?

I am actually planing to gain more flexibility in my experiment so if I fix the cells after staining them will it affect the surface expression of antibodies in anyway?

11 November 2017 1,184 4 View

How readily CD88, CD54 and CD11c are expressed on the surface of neutrophil ? In neutrophil from healthy blood only 10% cells are positive for CD88.

I isolated neutrophil from healthy blood and all of my neutrophils are positive for CD16, CD62L and CD11b antibody but only 60% of neutrophil are positive for CD11c, 71% for CD54 and only 10% for...

08 August 2017 1,807 3 View

How much time does acetone take for complete deflation of fish Viscera for Glycosaminoglycan extraction?

I am extracting glycosaminoglycan from fish viscera of Seabass and I am facing some difficulties, as I kept tissue in acetone for more than one month but still I am getting lots of fat and not...

08 August 2014 10,039 3 View