Ravi Kanth Reddy Sathi

8 Questions 12 Answers 0 Followers

Questions related from Ravi Kanth Reddy Sathi

What is the formula to calculate the critical value of correlation?

I am calculating the correlation values between two data sets of size 257. I want to know what is the critical value of correlation for a sample size of 257. I tried searching on the web, but...

13 December 2013 8,631 18 View

What is the minimum coverage and how to identity percentage for protein domains?

I am trying to find out existence of protein domains in a set of sequences. I am using BLASTX for the task. I have made a BLASTX of my sequences with the ProDom sequences. I used an e-value cutoff...

06 November 2013 3,844 3 View

How to screen genomes for compositional studies?

I am working with around 2600+ genomes and wish to study the genome, gene and intergenic features among various groups. In case of taxonomical groups which have very few representatives, there is...

27 September 2013 5,829 5 View

Should regulatory sequences be included in intergenic sequences?

I am working on finding the functional signatures/remains of regulatory regions, RNAs and proteins in the intergenic sequences (IGs) of E. coli K12 MG1655. I have a few doubts regarding the...

21 August 2013 3,691 1 View

How do I Rectify NCBI C++ Exception? ncbi::CMemoryFileSegment::CMemoryFileSegment()

I have tried running a command line blast. The query file is a multi fasta file containing 2600 sequences. It was made a BLASTX against a proteins sequences (ProDom) of size 2 GB (prodom.phr :...

11 August 2013 3,674 0 View

How efficient are PERL and PHP in designing bioinformatics tools? What are the pros and cons of each and which is most commonly used and why?

Out of my experience I think the basic difference among both of them lies in speed and usage. PHP can be used for creating online tools. PERL can also be used, but PHP is more easy to handle to...

24 July 2013 2,635 3 View

Are there any cloud computation facilities for Bioinformatics work?

Can some one please throw some light on cloud facilities available to carry on Bioinformatics work. Also is there a possibility of using such services for free or for a nominal fee, for academic...

07 July 2012 9,949 17 View

Logic flow for backtracking step in Needleman Wunsch algorithm?

I am trying to compile a code for running Needleman Wunsch algorithm using PHP. I could come up to matrix construction and filling. I don't understand how to write the logic for back tracking so...

08 February 2012 1,155 10 View