Raven Baxter-Christian

2 Questions 1 Answers 0 Followers

Questions related from Raven Baxter-Christian

Why isn't it counter-intuitive to include EDTA and MgCl2 in the same PCR mix?

I am new to PCR and digging into learning the roles of the different components in a PCR based on the FastStart HF PCR System I am using from Roche. If EDTA is a chelating agent, and we need the...

28 July 2016 754 4 View

Is it okay to rerun your sample through a genomic DNA isolation kit if the A260/A280 ratio was too low the first time?

I was given a tube of GFP DNA to use for a transfection and the quality of the DNA according to several repeats of readings on the NanoDrop is very poor. The A260/A280 ratio is around .6. Is it...

12 July 2016 3,554 6 View