What happened to my procedure to result in a very low yield of XOS production from rice bran?

My hemicellulose extraction step; 1) defatted rice bran 2) destarch by amylase (termamyl) 3) deprotein by NaOH at pH 11  4) alkali treatment for hemicellulose extraction by 1.25 N of NaOH for 24...

25 October 2015 4,102 2 View