Rathnavel Pandian Thangamani

4 Questions 5 Answers 0 Followers

Questions related from Rathnavel Pandian Thangamani

How can I prevent sections of adult drosophila brains from washing away from the slides during staining procedure?

I am working on 30um sections of adult drosophila brains. I dual stain the sections first with Alexa-fluor 594 anti-HRP stain and then with DAPI to stain the neurons and the nuclei...

14 December 2015 684 4 View

Can I stain a single specimen with both Golgi staining and DAPI? Are they compatible?

I am using Golgi's staining protocol to stain my neurons (adult drosophila brains - sectioned). Since I am studying the very minute retinal neurons, I am having difficulty in picking out their...

21 August 2015 2,134 3 View

How do I dissolve the tissue freezing medium after fixing the sectioned specimens onto my slide?

I have to do post-section staining of my slides. With the tissue freezing medium present, my specimens were washed away during staining. So, how do I dissolve this medium without affecting my...

13 August 2015 1,988 3 View

In what solution can an insect, along with its nervous system intact, be preserved for cryotome sectioning?

I'm studying an insect's brain and I would like to know what would be the ideal preserving solution to keep the nervous system of the insect intact. Will ethanol or formaldehyde solution be ideal?

22 October 2014 5,975 2 View