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I am going to determine the amount of lipids produced by a kind of yeast. However, because the culture medium produces lots of foam during aeration, I have to use a kind of anti foam. I would like...
30 May 2014 4,907 6 View
I am trying to calculate the specific growth rate for a species of Acetobacter. The substrate is glucose. Batch culture is used by regulating pH, temperature, and dissolved oxygen. According to...
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A bacterial strain has been grown under certain conditions, and catalase and superoxidase activity have been determined in exponential and stationary phase. During stationary phase, these enzyme...
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In bacterial physiology, lag phase is defined as the phase which is necessary for adaption of cells to new environment. During this phase the bacteria grow and the size increases; but the...
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Could you please clearly explain the extinction coefficient and its importance in enzymatic assays? Should we consider it for all type of enzyme assays?
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For calculation of the generations in broth medium, I determine the initial number of bacteria and then the final number of them in a given time. Afterwards, by using the formula, I can determine...
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I have used French press to disrupt bacterial cells. After ultracentrifugation, the supernatant was used for determination of enzyme activity (PQQ-Glucose dehydrogenase). Now, I would like to...
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