Rasoul Shafiei

6 Questions 30 Answers 0 Followers

Questions related from Rasoul Shafiei

How can I calculate the specific growth rate and lag phase?

I am trying to calculate the specific growth rate for a species of Acetobacter. The substrate is glucose. Batch culture is used by regulating pH, temperature, and dissolved oxygen. According to...

20 March 2014 4,238 53 View

How can I determine that a cell population is under oxidative stress?

A bacterial strain has been grown under certain conditions, and catalase and superoxidase activity have been determined in exponential and stationary phase. During stationary phase, these enzyme...

11 September 2013 1,195 26 View

Is the amount of inoculum important to decrease the lag phase? How does it help each bacterial cell to decrease the time needed for getting ready?

In bacterial physiology, lag phase is defined as the phase which is necessary for adaption of cells to new environment. During this phase the bacteria grow and the size increases; but the...

06 August 2013 1,195 41 View

What is the Extinction coefficient that is used in the determination of enzyme activity ?

Could you please clearly explain the extinction coefficient and its importance in enzymatic assays? Should we consider it for all type of enzyme assays?

25 July 2013 5,786 6 View

How can I determine the number of bacterial generations in solid culture media?

For calculation of the generations in broth medium, I determine the initial number of bacteria and then the final number of them in a given time. Afterwards, by using the formula, I can determine...

18 July 2013 3,467 9 View

Can I use cell crude extract to determine the effect of an inhibitory compound on one of the enzymes?

I have used French press to disrupt bacterial cells. After ultracentrifugation, the supernatant was used for determination of enzyme activity (PQQ-Glucose dehydrogenase). Now, I would like to...

15 July 2013 5,067 10 View