Rashmi Kulkarni

3 Questions 23 Answers 0 Followers

Questions related from Rashmi Kulkarni

My C2C12 myotubes are coming off of the 96 wells plate, do you have any suggestions how I can control this?

I am growing myotubes in 96 well plate for treatment with free fatty acid. However, they come off after washing or even treatment with some dye for 15-20 minutes in a buffer. This is with myotubes...

05 May 2015 4,031 9 View

How is MitoTEMPO treatment given? Added along with the stress inducer or separately after the assay?

I am treating C2C1 myotubes with free fatty acid and would like to measure mitochondrial specific super-oxide production. I will be using MitoTEMPO for specificity. I have couple of questions...

03 March 2015 9,020 17 View

How reliable is MitosoxRed for measurement in 96 well plates?

I would like to use Mitosox red o read mitochondrial ROS. I have seen that mostly it is used for imaging purposes. How reliable it is to use it in 96 wells plate assay mode? If somebody has...

09 September 2014 4,294 12 View