Rashmi Gangwar

2 Questions 3 Answers 0 Followers

Questions related from Rashmi Gangwar

Can use RppH enzyme for dephosphorylation and decapping at once?

I am dephosphorylation RNA by Antarctic phosphatase and then decapping it by RppH (NEB). I am curious to know can i only use Rpph for for the process in one single reaction.As i have read RppH can...

21 March 2017 1,718 2 View

I am doing 5' RACE using RLM RACE kit Ist pcr no band was observed but in nested pcr i see smear in lane. Can anyone help?

nestd pcr gel image

07 March 2017 8,825 3 View